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Here are some of the great features of Mo-Bio 12224-250 1508 Piece UltraClean Microbial DNA Isolation Kit, 250 Preps
Mo-Bio 12224-250 1508, The UltraClean microbial DNA Kit is designed to isolate high-quality genomic DNA from microorganisms. A variety of microorganisms including bacterial spores and fungal types have been tested successfully with this kit. Microbial cells are added to a tube containing beads, bead solution and lysis solution. The prinicipal is to lyse the microorganisms by a combination of heat, detergent and mechanical force against specialized beads. The cellular components are lysed by using a specially designed mo bio vortex adapter on a standard vortex. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free tris buffer. Format: spin filter method. Throughput: 1-24 samples. Yield: up to 20 microgram. Time: 20 minutes. Required: mo bio vortex adapter, micro centrifuge. Kit includes: 1 x 15ml solution MD1, 1 x 30ml solution MD2, 1 x 250ml solution MD3, 3 x 25ml solution MD4, 1 x 15ml solution MD5, 250 microbead solution, 1 x 80ml microbead tubes, 250 spin filters in tubes, 1000 collection tubes.
- No hazardous organic solvents or enzymes required
- Yields DNA greater than 50 kb in length from yeast, fungi, gram negative and gram positive bacteria
- Silica spin filter method, 20-minute protocol (total time)
- DNA is ready for PCR, restriction digests, all downstream applications
- All kits are ready-to-use and include all reagents, spin filters and tubes
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